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jnk  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc jnk
    Jnk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 98/100, based on 7558 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/sapk+jnk+antibody/SAPK%2FJNK+Antibody/pm41680474-70-107-110
    Average 98 stars, based on 7558 article reviews
    jnk - by Bioz Stars, 2026-10
    98/100 stars

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    Article Title: Probiotics-enhanced kynurenic acid mitigates cisplatin-induced nephrotoxicity in mice
    Article Snippet: SAPK/JNK Antibody , CST , Cat# 9252, RRID: AB_2250373.

    TUNEL Assay:

    Article Title: Extracellular Vesicles of Streptococcus anginosus Mediate Gastritis via Epithelial Barrier Disruption and Macrophage‐driven Inflammation
    Article Snippet: Anti‐mouse IL‐17A antibody (Cat: 506901), anti‐mouse CCL‐20 antibody (Cat: 741299), anti‐mouse TNF‐a antibody (Cat: 506301), anti‐mouse IL‐1β antibody (Cat: 740865), anti‐mouse CCL‐8 antibody (Cat: 741072), anti‐mouse IL‐6 antibody (Cat: 504509), anti‐mouse IL‐10 antibody (Cat: 505029), anti‐mouse IL‐22 antibody (Cat: 740738) were purchased from Biolegend (Guangzhou, China). .. Anti‐Ki67 Rabbit pAb (Cat: GB111499 ‐50), Anti‐PCNA Rabbit pAb (Cat: GB11010‐50), TUNEL Apoptosis Detection Kit (CF488 Green fluorescence) (Cat: G1504‐50T), Anti‐ZO1 tight junction protein Rabbit pAb (Cat: GB111981 ‐50), Anti‐Claudin18 Rabbit pAb (Cat: GB114352 ‐50), Anti‐Occludin Rabbit pAb (Cat: GB111401 ‐50) were purchased from BD Servicebio (Guangzhou, China), Akt antibody (Cat: 9272S), Phospho (Ser/Thr) Akt Substrate antibody (Cat: 8611L), p44/42 MAPK (Erk1/2) antibody (Cat: 4668), Phospho‐p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat: 4671), SAPK/JNK antibody (Cat: 9252), phospho‐SAPK/JNK (Thr183/Tyr185) antibody (Cat: 9251), Toll‐like Receptor 2 antibody (Cat: 8206), GAPDH (D4C6R) mouse mAb (Cat: 9251) were purchased from Cell Signaling Technology (Shanghai, China). ..

    Article Title: Extracellular Vesicles of Streptococcus anginosus Mediate Gastritis via Epithelial Barrier Disruption and Macrophage-driven Inflammation.
    Article Snippet: Anti-mouse IL-17A antibody (Cat: 506901), anti-mouse CCL-20 antibody (Cat: 741299), anti-mouse TNF-a antibody (Cat: 506301), anti-mouse IL-1 β antibody (Cat: 740865), anti-mouse CCL-8 antibody (Cat: 741072), anti-mouse IL-6 antibody (Cat: 504509), anti-mouse IL-10 antibody (Cat: 505029), antimouse IL-22 antibody (Cat: 740738) were purchased from Biolegend (Guangzhou, China). .. Anti-Ki67 Rabbit pAb (Cat: GB111499- Advanced Science, 2026 50), Anti-PCNA Rabbit pAb (Cat: GB11010-50), TUNEL Apoptosis Detection Kit (CF488 Green fluorescence) (Cat: G1504-50T), Anti-ZO1 tight junction protein Rabbit pAb (Cat: GB111981-50), Anti-Claudin18 Rabbit pAb (Cat: GB114352-50), Anti-Occludin Rabbit pAb (Cat: GB111401-50) were purchased from BD Servicebio (Guangzhou, China), Akt antibody (Cat: 9272S), Phospho (Ser/Thr) Akt Substrate antibody (Cat: 8611L), p44/42 MAPK (Erk1/2) antibody (Cat: 4668), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat: 4671), SAPK/JNK antibody (Cat: 9252), phospho-SAPK/JNK (Thr183/Tyr185) antibody (Cat: 9251), Toll-like Receptor 2 antibody (Cat: 8206), GAPDH (D4C6R) mouse mAb (Cat: 9251) were purchased from Cell Signaling Technology (Shanghai, China). ..

    Fluorescence:

    Article Title: Extracellular Vesicles of Streptococcus anginosus Mediate Gastritis via Epithelial Barrier Disruption and Macrophage‐driven Inflammation
    Article Snippet: Anti‐mouse IL‐17A antibody (Cat: 506901), anti‐mouse CCL‐20 antibody (Cat: 741299), anti‐mouse TNF‐a antibody (Cat: 506301), anti‐mouse IL‐1β antibody (Cat: 740865), anti‐mouse CCL‐8 antibody (Cat: 741072), anti‐mouse IL‐6 antibody (Cat: 504509), anti‐mouse IL‐10 antibody (Cat: 505029), anti‐mouse IL‐22 antibody (Cat: 740738) were purchased from Biolegend (Guangzhou, China). .. Anti‐Ki67 Rabbit pAb (Cat: GB111499 ‐50), Anti‐PCNA Rabbit pAb (Cat: GB11010‐50), TUNEL Apoptosis Detection Kit (CF488 Green fluorescence) (Cat: G1504‐50T), Anti‐ZO1 tight junction protein Rabbit pAb (Cat: GB111981 ‐50), Anti‐Claudin18 Rabbit pAb (Cat: GB114352 ‐50), Anti‐Occludin Rabbit pAb (Cat: GB111401 ‐50) were purchased from BD Servicebio (Guangzhou, China), Akt antibody (Cat: 9272S), Phospho (Ser/Thr) Akt Substrate antibody (Cat: 8611L), p44/42 MAPK (Erk1/2) antibody (Cat: 4668), Phospho‐p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat: 4671), SAPK/JNK antibody (Cat: 9252), phospho‐SAPK/JNK (Thr183/Tyr185) antibody (Cat: 9251), Toll‐like Receptor 2 antibody (Cat: 8206), GAPDH (D4C6R) mouse mAb (Cat: 9251) were purchased from Cell Signaling Technology (Shanghai, China). ..

    Article Title: Extracellular Vesicles of Streptococcus anginosus Mediate Gastritis via Epithelial Barrier Disruption and Macrophage-driven Inflammation.
    Article Snippet: Anti-mouse IL-17A antibody (Cat: 506901), anti-mouse CCL-20 antibody (Cat: 741299), anti-mouse TNF-a antibody (Cat: 506301), anti-mouse IL-1 β antibody (Cat: 740865), anti-mouse CCL-8 antibody (Cat: 741072), anti-mouse IL-6 antibody (Cat: 504509), anti-mouse IL-10 antibody (Cat: 505029), antimouse IL-22 antibody (Cat: 740738) were purchased from Biolegend (Guangzhou, China). .. Anti-Ki67 Rabbit pAb (Cat: GB111499- Advanced Science, 2026 50), Anti-PCNA Rabbit pAb (Cat: GB11010-50), TUNEL Apoptosis Detection Kit (CF488 Green fluorescence) (Cat: G1504-50T), Anti-ZO1 tight junction protein Rabbit pAb (Cat: GB111981-50), Anti-Claudin18 Rabbit pAb (Cat: GB114352-50), Anti-Occludin Rabbit pAb (Cat: GB111401-50) were purchased from BD Servicebio (Guangzhou, China), Akt antibody (Cat: 9272S), Phospho (Ser/Thr) Akt Substrate antibody (Cat: 8611L), p44/42 MAPK (Erk1/2) antibody (Cat: 4668), Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) antibody (Cat: 4671), SAPK/JNK antibody (Cat: 9252), phospho-SAPK/JNK (Thr183/Tyr185) antibody (Cat: 9251), Toll-like Receptor 2 antibody (Cat: 8206), GAPDH (D4C6R) mouse mAb (Cat: 9251) were purchased from Cell Signaling Technology (Shanghai, China). ..

    MTT Assay:

    Article Title: Identifying the effective components of Yaobitong capsule against lumbar disc herniation using a multi-technique strategy.
    Article Snippet: The Yaobitong Capsule (YBTC) is a notable traditional Chinese medicine employed in the treatment of lumbar disc herniation (LDH).. However, its therapeutic effective components are not well understood.. This study aimed to systematically elucidate its pharmacodynamic basis by integrating comprehensive chemical analysis, bioactivity prediction, and experimental validation.

    RNA Extraction:

    Article Title: Identifying the effective components of Yaobitong capsule against lumbar disc herniation using a multi-technique strategy.
    Article Snippet: The Yaobitong Capsule (YBTC) is a notable traditional Chinese medicine employed in the treatment of lumbar disc herniation (LDH).. However, its therapeutic effective components are not well understood.. This study aimed to systematically elucidate its pharmacodynamic basis by integrating comprehensive chemical analysis, bioactivity prediction, and experimental validation.

    Isolation:

    Article Title: Identifying the effective components of Yaobitong capsule against lumbar disc herniation using a multi-technique strategy.
    Article Snippet: The Yaobitong Capsule (YBTC) is a notable traditional Chinese medicine employed in the treatment of lumbar disc herniation (LDH).. However, its therapeutic effective components are not well understood.. This study aimed to systematically elucidate its pharmacodynamic basis by integrating comprehensive chemical analysis, bioactivity prediction, and experimental validation.

    Phospho-proteomics:

    Article Title: Oxygen-dependent regulation of ADAMTS1 by VEGFA defines a novel VEGFA-HIF-ADAMTS1 axis in hepatocellular cancer.
    Article Snippet: ADAMTS1 is a matrix-associated protease involved in angiogenesis and tumor microenvironment remodeling, and its regulation under oxygen-limited conditions has not been fully elucidated.. Vascular endothelial growth factor A (VEGFA) is one of the key regulators of angiogenesis and hypoxic signaling.. This study aimed to reveal the effects of VEGFA on ADAMTS1 gene expression, promoter activity, and related signaling pathways under normoxic and hypoxic conditions.

    In Vitro:

    Article Title: Chemically-induced degradation of the endoplasmic-reticulum stress sensor IRE1 by a VHL-recruiting chimera
    Article Snippet: Signal was detected using appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies at 1:10,000 in 5% milk at room temperature for 1–2 h. Bands were visualized by the Azure 600 western blot imaging system (Azure Biosystems). .. For in vitro ubiquitination assays performed with Me-Ub, bands were visualized by the Odyssey CLx Imager (LI‐COR) after incubating the blots with the IRDye 800CW (LI-COR) secondary antibodies at 1:10,000 in Intercept Blocking Buffer (LI-COR) at room temperature for 1–2 h. Primary antibodies used in the studies: IRE1α (14C10) Rabbit mAb (Cell Signaling, #3294), GAPDH (14C10) Rabbit mAb (Cell Signaling, #2118), CDK7 Antibody (Cell Signaling, #2090), CDK9 (C12F7) Rabbit mAb (Cell Signaling, #2316), GSK-3α (D80E6) Rabbit mAb (Cell Signaling, #4337), CSF-1R/M-CSF-R Antibody (Cell Signaling, #3152), SAPK/JNK Antibody (Cell Signaling, #9252), JNK2 (56G8) Rabbit mAb (Cell Signaling, #9258), JNK3 (55A8) Rabbit mAb (Cell Signaling, #2305), MELK Antibody (Cell Signaling, #2274), PKD/PKCμ (D4J1N) Rabbit mAb (Cell Signaling, #90039), TAO2 antibody (Novus Biologicals, NBP2-20563), K48-linkage Specific Polyubiquitin Antibody (Cell Signaling, # 4289), VHL Antibody (Cell Signaling, #68547), LC3A/B (D3U4C) XP® Rabbit mAb (Cell Signaling, #12741), p-IRE1 antibody (made in-house at Genentech, described in Chang et al. ), K11 linkage-specific antibody (made in-house at Genentech, described in Matsumoto et al. ). .. Secondary antibodies used in the studies: Peroxidase AffiniPureTM Donkey Anti-Rabbit IgG (H+L) (Jackson Immuno Research Laboratories, #711-035-152), Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson Immuno Research Laboratories, #115-035-003), Goat Anti-Mouse IgG2a Human ads-HRP (SouthernBiotech, #1080-05), IRDye® 800CW Goat Anti-Rabbit IgG (LI-COR, #926-32211).

    Ubiquitin Proteomics:

    Article Title: Chemically-induced degradation of the endoplasmic-reticulum stress sensor IRE1 by a VHL-recruiting chimera
    Article Snippet: Signal was detected using appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies at 1:10,000 in 5% milk at room temperature for 1–2 h. Bands were visualized by the Azure 600 western blot imaging system (Azure Biosystems). .. For in vitro ubiquitination assays performed with Me-Ub, bands were visualized by the Odyssey CLx Imager (LI‐COR) after incubating the blots with the IRDye 800CW (LI-COR) secondary antibodies at 1:10,000 in Intercept Blocking Buffer (LI-COR) at room temperature for 1–2 h. Primary antibodies used in the studies: IRE1α (14C10) Rabbit mAb (Cell Signaling, #3294), GAPDH (14C10) Rabbit mAb (Cell Signaling, #2118), CDK7 Antibody (Cell Signaling, #2090), CDK9 (C12F7) Rabbit mAb (Cell Signaling, #2316), GSK-3α (D80E6) Rabbit mAb (Cell Signaling, #4337), CSF-1R/M-CSF-R Antibody (Cell Signaling, #3152), SAPK/JNK Antibody (Cell Signaling, #9252), JNK2 (56G8) Rabbit mAb (Cell Signaling, #9258), JNK3 (55A8) Rabbit mAb (Cell Signaling, #2305), MELK Antibody (Cell Signaling, #2274), PKD/PKCμ (D4J1N) Rabbit mAb (Cell Signaling, #90039), TAO2 antibody (Novus Biologicals, NBP2-20563), K48-linkage Specific Polyubiquitin Antibody (Cell Signaling, # 4289), VHL Antibody (Cell Signaling, #68547), LC3A/B (D3U4C) XP® Rabbit mAb (Cell Signaling, #12741), p-IRE1 antibody (made in-house at Genentech, described in Chang et al. ), K11 linkage-specific antibody (made in-house at Genentech, described in Matsumoto et al. ). .. Secondary antibodies used in the studies: Peroxidase AffiniPureTM Donkey Anti-Rabbit IgG (H+L) (Jackson Immuno Research Laboratories, #711-035-152), Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson Immuno Research Laboratories, #115-035-003), Goat Anti-Mouse IgG2a Human ads-HRP (SouthernBiotech, #1080-05), IRDye® 800CW Goat Anti-Rabbit IgG (LI-COR, #926-32211).

    Blocking Assay:

    Article Title: Chemically-induced degradation of the endoplasmic-reticulum stress sensor IRE1 by a VHL-recruiting chimera
    Article Snippet: Signal was detected using appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies at 1:10,000 in 5% milk at room temperature for 1–2 h. Bands were visualized by the Azure 600 western blot imaging system (Azure Biosystems). .. For in vitro ubiquitination assays performed with Me-Ub, bands were visualized by the Odyssey CLx Imager (LI‐COR) after incubating the blots with the IRDye 800CW (LI-COR) secondary antibodies at 1:10,000 in Intercept Blocking Buffer (LI-COR) at room temperature for 1–2 h. Primary antibodies used in the studies: IRE1α (14C10) Rabbit mAb (Cell Signaling, #3294), GAPDH (14C10) Rabbit mAb (Cell Signaling, #2118), CDK7 Antibody (Cell Signaling, #2090), CDK9 (C12F7) Rabbit mAb (Cell Signaling, #2316), GSK-3α (D80E6) Rabbit mAb (Cell Signaling, #4337), CSF-1R/M-CSF-R Antibody (Cell Signaling, #3152), SAPK/JNK Antibody (Cell Signaling, #9252), JNK2 (56G8) Rabbit mAb (Cell Signaling, #9258), JNK3 (55A8) Rabbit mAb (Cell Signaling, #2305), MELK Antibody (Cell Signaling, #2274), PKD/PKCμ (D4J1N) Rabbit mAb (Cell Signaling, #90039), TAO2 antibody (Novus Biologicals, NBP2-20563), K48-linkage Specific Polyubiquitin Antibody (Cell Signaling, # 4289), VHL Antibody (Cell Signaling, #68547), LC3A/B (D3U4C) XP® Rabbit mAb (Cell Signaling, #12741), p-IRE1 antibody (made in-house at Genentech, described in Chang et al. ), K11 linkage-specific antibody (made in-house at Genentech, described in Matsumoto et al. ). .. Secondary antibodies used in the studies: Peroxidase AffiniPureTM Donkey Anti-Rabbit IgG (H+L) (Jackson Immuno Research Laboratories, #711-035-152), Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson Immuno Research Laboratories, #115-035-003), Goat Anti-Mouse IgG2a Human ads-HRP (SouthernBiotech, #1080-05), IRDye® 800CW Goat Anti-Rabbit IgG (LI-COR, #926-32211).

    Clinical Proteomics:

    Article Title: Chemically-induced degradation of the endoplasmic-reticulum stress sensor IRE1 by a VHL-recruiting chimera
    Article Snippet: Signal was detected using appropriate horseradish peroxidase (HRP)-conjugated secondary antibodies at 1:10,000 in 5% milk at room temperature for 1–2 h. Bands were visualized by the Azure 600 western blot imaging system (Azure Biosystems). .. For in vitro ubiquitination assays performed with Me-Ub, bands were visualized by the Odyssey CLx Imager (LI‐COR) after incubating the blots with the IRDye 800CW (LI-COR) secondary antibodies at 1:10,000 in Intercept Blocking Buffer (LI-COR) at room temperature for 1–2 h. Primary antibodies used in the studies: IRE1α (14C10) Rabbit mAb (Cell Signaling, #3294), GAPDH (14C10) Rabbit mAb (Cell Signaling, #2118), CDK7 Antibody (Cell Signaling, #2090), CDK9 (C12F7) Rabbit mAb (Cell Signaling, #2316), GSK-3α (D80E6) Rabbit mAb (Cell Signaling, #4337), CSF-1R/M-CSF-R Antibody (Cell Signaling, #3152), SAPK/JNK Antibody (Cell Signaling, #9252), JNK2 (56G8) Rabbit mAb (Cell Signaling, #9258), JNK3 (55A8) Rabbit mAb (Cell Signaling, #2305), MELK Antibody (Cell Signaling, #2274), PKD/PKCμ (D4J1N) Rabbit mAb (Cell Signaling, #90039), TAO2 antibody (Novus Biologicals, NBP2-20563), K48-linkage Specific Polyubiquitin Antibody (Cell Signaling, # 4289), VHL Antibody (Cell Signaling, #68547), LC3A/B (D3U4C) XP® Rabbit mAb (Cell Signaling, #12741), p-IRE1 antibody (made in-house at Genentech, described in Chang et al. ), K11 linkage-specific antibody (made in-house at Genentech, described in Matsumoto et al. ). .. Secondary antibodies used in the studies: Peroxidase AffiniPureTM Donkey Anti-Rabbit IgG (H+L) (Jackson Immuno Research Laboratories, #711-035-152), Peroxidase-AffiniPure Goat Anti-Mouse IgG (H+L) (Jackson Immuno Research Laboratories, #115-035-003), Goat Anti-Mouse IgG2a Human ads-HRP (SouthernBiotech, #1080-05), IRDye® 800CW Goat Anti-Rabbit IgG (LI-COR, #926-32211).



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